Genetic diversity and dynamics of Sinorhizobium meliloti populations nodulating different alfalfa cultivars in Italian soils.
نویسندگان
چکیده
We analyzed the genetic diversity of 531 Sinorhizobium meliloti strains isolated from nodules of Medicago sativa cultivars in two different Italian soils during 4 years of plant growth. The isolates were analyzed for DNA polymorphism with the random amplified polymorphic DNA method. The populations showed a high level of genetic polymorphism distributed throughout all the isolates, with 440 different haplotypes. Analysis of molecular variance allowed us to relate the genetic structure of the symbiotic population to various factors, including soil type, alfalfa cultivar, individual plants within a cultivar, and time. Some of these factors significantly affected the genetic structure of the population, and their relative influence changed with time. At the beginning of the experiment, the soil of origin and, even more, the cultivar significantly influenced the distribution of genetic variability of S. meliloti. After 3 years, the rhizobium population was altered; it showed a genetic structure based mainly on differences among plants, while the effects of soil and cultivar were not significant.
منابع مشابه
پراکنش گونههای سینوریزوبیومی Sinorhizobium meliloti و S. medicae همزیست با یونجه در مناطق غربی ایران
To characterize the geographical distribution of medicago-nodulating rhizobia in western regions of Iran, 950 Sinorhizobium isolates were trapped from a combination of two local alfalfa populations (Hamedani, Nikshahri) together with a foreign cultivar ( Kodi) and soil samples from eight sites across Kurdestan, Kermanshah, Eastern Azarbayjan and Lorestan provinces. Also, a total of 45 isolates ...
متن کاملپراکنش گونههای سینوریزوبیومی Sinorhizobium meliloti و S. medicae همزیست با یونجه در مناطق غربی ایران
To characterize the geographical distribution of medicago-nodulating rhizobia in western regions of Iran, 950 Sinorhizobium isolates were trapped from a combination of two local alfalfa populations (Hamedani, Nikshahri) together with a foreign cultivar ( Kodi) and soil samples from eight sites across Kurdestan, Kermanshah, Eastern Azarbayjan and Lorestan provinces. Also, a total of 45 isolates ...
متن کاملمطالعه تنوع ژنتیک باکتریهای سینوریزوبیوم با استفاده از تکنیک PCR / RFLP 16S-23S rDNA
It is important to investigate the genetic diversity and evaluate symbiotic effectiveness of the indigenous rhizobial population. It helps understand the responses of indigenous isolates to different rhizobial inoculants. In spite of the importance of bacterial diversity, there are a few scientific reports about it in Iranian soils. Genetic diversity of 150 isolates of Sinorhizobium isolated ...
متن کاملمطالعه تنوع ژنتیک باکتریهای سینوریزوبیوم با استفاده از تکنیک PCR / RFLP 16S-23S rDNA
It is important to investigate the genetic diversity and evaluate symbiotic effectiveness of the indigenous rhizobial population. It helps understand the responses of indigenous isolates to different rhizobial inoculants. In spite of the importance of bacterial diversity, there are a few scientific reports about it in Iranian soils. Genetic diversity of 150 isolates of Sinorhizobium isolated ...
متن کاملErwinia herbicola isolates from alfalfa plants may play a role in nodulation of alfalfa by Rhizobium meliloti.
Erwinia herbicola was isolated from roots of plants derived from surface-sterilized seeds of all alfalfa varieties that were tested. Some of these E. herbicola strains affected nodulation by certain strains of Rhizobium meliloti. In previously published work we presented the isolation of slow-and fast-nodulating variants from a single culture of R. meliloti 102F51. In the absence of E. herbicol...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 66 11 شماره
صفحات -
تاریخ انتشار 2000